I would like to dectect phosphoproteins directly on acrylamide gels. I woder if I could use stain free gels from BioRad which detect total loaded protein to stain them with pro-q diamond.
Yes, the Stain-Free gels from BioRad are compatible with Pro-Q Diamond. (I emailed BioRad to ask, and they said yes; I also just finished running/staining a gel, and it turned out fine.)