Krishnashish Bose

10 Questions 52 Answers 0 Followers

Questions related from Krishnashish Bose

Do you know of Physicists who switched to Neuroscience after PhD?

I wonder how can a Physicist work with live animals as a PI without collaborating with biologists.

07 July 2018 7,886 8 View

How to make electrical contact with several micro-electrodes of just 10 micron width at the tip of a fiber of just ~0.15 mm diameter?

The electrodes are used for voltage measurement in the Brain and needs to be connected to pre-amplifiers. Is there any company or research group that can fabricate transparent microchip for this...

10 October 2017 2,060 8 View

Do you think height differences of 0.3 - 1 nm is significant enough for comparison using ScanAsyst mode?

I have different samples with height in the range of about 1.5 to 3 nm; as measured by ScanAsyst mode in air, with same AFM probe and same imaging force. I know that height measured by tapping...

01 January 2017 2,158 5 View

Is there a way to visualize molecules using VMD without any light and shadows?

I have colored the molecule with b-factor and I don't want any lighting or shadows to influence this coloring.

04 April 2016 1,217 3 View

Is there any software available to fit a cylinder to a set of 3d coordinates?

I am trying to estimate the width of DNA from its crystal structure.

09 September 2014 9,875 6 View

Does anyone know about deflection sensitivity correction factor?

For force spectroscopy experiments with AFM, it is needed to calibrate the deflection sensitivity and spring constant of the cantilever. First I determine the deflection sensitivity very...

05 May 2014 4,728 17 View

Is it possible to measure the contour length of features from an image?

I am imaging DNA using AFM. I extracted the gray scale intensity values from the image into a matrix of integer elements. Then I wrote a code in C++ to count pixels falling within a certain...

03 March 2014 5,044 28 View

Can someone suggest the best way to immobilize single stranded DNA and G quadruplexes on Mica for AFM imaging in water?

Other than the usual method of adding divalent cations of small ionic radii, is there any better method? I want very clean background.

11 November 2013 6,987 0 View

What is the best vibration isolation solution for AFM?

Are the isolation tables using nitrogen or compressed air supply the best? What do you think about the active isolation platforms available from Herzan and Accurion that runs simply on electricity?

10 October 2013 4,428 13 View

Is it possible to do transcription with the circular plasmid, instead of linearizing it?

Two special cases: 1. Both the promoter & terminator sequence is present. 2. Only the promoter sequence is present.

01 January 1970 6,638 3 View