Where can I find the scoring key for The Profile of Emotional Competence?

The author of this instrument has provided an email address in which to request a copy of the scoring key. However, I have attempted to contact him several times without success. I have been...

08 December 2019 5,289 1 View

Why am I unable to reproduce PCR genotyping results for a single colony that previously produced a band but now doesn't?

Previously I genotyped a 96-well plate containing genomic DNA from cell lysate. I was able to get a band at the desired location, however I am trying to reproduce the genotyping results for a...

28 August 2019 3,648 3 View

I am in need of a questionnaire on children's emotional development that is completed by their parent. Does anyone know of such an instrument?

I am looking for a survey or questionnaire on a child's emotional development that their parent completes. A survey/questionnaire on emotional growth, emotional competence, emotional well-being,...

03 May 2018 2,361 2 View

Current projects that involve collaboration between the arts and sciences?

I am looking for references to current projects that involve collaboration between the arts and sciences as part of my Future Fellowship research. If you are working in this area, or know of any...

02 February 2018 8,249 2 View

How trustworthy is evidence based research as applied in the natural sciences and the social sciences?

In medicine, I think evidence-based research and - crucial, this - evidence-based practice is quite robust. Insofar as social scientific veracity is concerned, the wide range of variables does...

06 September 2017 4,948 5 View

Does anyone have a preferred spectrophotometric detection method for arsenite vs. arsenate?

So far we have tried a variamine blue assay from Narayana et al. 2006 and a silver nitrate precipitation from Simeonova et al. 2004. In the variamine blue assay instead of intensity correlating...

29 June 2016 1,373 2 View

Order of minigene-GFP construct for IVT and electroporation for human dendritic cells?

We are developing a GFP construct to use as a marker for successful electroporation of a minigene into human dendritic cells. the purpose is to use the dendritic cells as potential targets for t...

28 January 2014 4,424 1 View

What is the best isotype for assessing background for cell surface activation/phenotyping markers by FACS?

I'm doing some extensive phenotyping by FACS of human lymphocyte populations. I'm looking at a number of markers- 41BB CD137 OX40 CD134 PD1 CTLA4 TIM3.... For many of these markers the isotype...

19 January 2014 8,114 3 View

What is the difference in effects of Bacteriophage and Antibiotics therapy?

Does anybody know of recent or current comparison studies between the effects of bacteriophage therapy and that with antibiotics?

03 October 2013 9,084 3 View

What is your favorite scientific paper of all time?

I am helping to teach a course on communicating science and through this course, I have come into contact with an absolutely beautiful paper. It is clear, concise, and elegant. I am not at all...

20 October 2012 558 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View