Can someone suggest a source where to get 5 keto fructose as a standard in HPLC from?

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Optimal condition for depositing FTO target 95:5% using sputter technique?

Hello I want to know about the sputtering condition of depositing FTO from target 95:5%. I tried with RF sputter in pressure 2.5Pa and 100 sccm Ar at room temperature but it showing no...

21 July 2024 1,680 2 View

How to extract good quality RNA from Plant parasitic nematodes?

Hi, I am dealing with plant parasitic nematodes. I tried extracting RNA from almost 1000J2 worms with two different kits (Qiagen RNeasy Kit, Omega RNA extraction Kit) and by two different ways of...

09 July 2024 3,742 2 View

ROS detection in microalgae?

Hi all, I need to measure ROS in microalgae to study the effect of autophagy. Can anyone suggest me a kit or method? I find only tests to be done in vitro. Thanks

20 April 2024 1,904 1 View

What is the one thing you would change about academic leadership?

There is a hierarchical structure in academic leadership which often favours the ones with most successful grants or fame in their own field. But leadership is a different ball game. Should...

05 April 2024 7,071 7 View

Is there any application, software or website that i can use for generating plagiarism reports, against my research, without involving a university?

In case one is not registered with a university?

25 March 2024 6,126 1 View

An inversion on a chromosome is detected, what are possible outcomes with a normal wife with normal karyotype? What additional information we need?

Background: A novel chromosomal inversion was identified in a phenotypically typical (live and fertile ) husband. The wife’s karyotype is normal. The couple is seeking genetic consultation...

02 March 2024 3,122 3 View

Researching Microalgae Selenoproteins: Where to Start?

I'm interested in researching microalgae selenoproteins but feel overwhelmed with where to begin. I'm seeking advice from the community on the best approaches and techniques for studying...

25 February 2024 4,371 0 View

How can we determine the sample size from an unknown population?

I want to calculate the sample size for my study on the education of Nomadic children in Lahore Pakistan. But no data regarding population size is available. How i can calculate and also give me...

02 February 2024 1,655 3 View

How I can perform Multicollinearity Test on GIS raster data?

I am using about 20 different variables (in the form of GIS layers/Raster). Before proceeding with the modeling, I want to perform collinearity test. Does anyone know how can I perform this test?...

11 January 2024 6,283 3 View

Why Men having vegetarian diet are more prone to Varicose veins than Men having Omnivorous diet?

Hi, I am reading a research article "The association between vegetarian diet and varicose veins might be more prominent in men than in women" .The overall result is that women have more varicose...

03 November 2023 337 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View