Nageena Zahid

7 Questions 10 Answers 0 Followers

Questions related from Nageena Zahid

How to extract good quality RNA from Plant parasitic nematodes?

Hi, I am dealing with plant parasitic nematodes. I tried extracting RNA from almost 1000J2 worms with two different kits (Qiagen RNeasy Kit, Omega RNA extraction Kit) and by two different ways of...

10 July 2024 3,698 2 View

Can someone suggest a source where to get 5 keto fructose as a standard in HPLC from?

I need 5 keto fructose as standard in HPLC. I tried different companies but not successful to buy it. Can anyone send me some link from where I can buy it easily.

04 June 2014 3,438 2 View

Does anyone knows about expressing two proteins in bacterial periplasm under one promoter and one signal peptide in one vector?

I have a plasmid with a strong promoter and a gene with a signal peptide. It works perfectly so far for expression. Now I want to clone one more gene in the same plasmid under the same promoter...

15 May 2014 2,565 3 View

What could be the possible reason to avoid osmotic stress by bacteria?

I have PEG in bacterial culture as osmoticum. I find the difference of half in term of O.D of stress versus control culture. On dry mass, there was difference of 10-15mg/100ml culture. Substrate...

12 May 2014 314 4 View

How can we calculate mosm/l for 10% polyethylene glycol(6000) in media?

I want to calculate osmolarity of 10%PEG in media (yeast extract 0,6%, Mannitol 2%). I want to make a hypotonic buffer to get compatible solutes from bacterial pellet by milking. I need mosm/l of...

05 May 2014 8,933 4 View

RIN Value of Bacterial RNA is 7 but 23/16S rRNA ratio is just 0.1?

I have isolated total RNA from gluconobacter oxydans. I want to do microarray analysis of my control and treated RNA samples . I have checked my samples on Bioanalyzer. The RIN value of my both...

19 July 2013 8,923 3 View

How to remove OCT efficiently from cryosections? How can we avoid the rolling of sections?

I am using Arabidopsis root tissues for longitudinal sections. When I tried to use series of ethanol and xylene to remove OCT from sections of membrane slide, the OCT is not removed completely....

26 June 2013 6,305 4 View