Can someone suggest a protocol to fix erythrocyte for FACS?

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Free software for deconvolution of mixtures in spectroscopic data?

Is there a free software to perform simulations and to deconvolute mixtures of compounds in FORS and Raman data? I would like to: - simulate mixtures of pigments and colorants with FORS spectra -...

15 April 2024 6,393 0 View

Wich RNAlater is better: from Thermo or Sigma?

I'm gonna start to colect biopsy samples from breast cancer and, in order to preserved the RNA for some hours until I can store the sample in the freezer, I'll use some RNAlater. However don't...

29 February 2024 6,147 3 View

Can you suggest literature criticizing the potential offensive implications of the "Neo-tribe" theory (Maffesoli, Cova & Cova, Hardy, etc.)?

Recently, I received peer review feedback that criticized my usage of terms like "neo-tribe" and "neo-tribalism" in a sociological paper. The reviewer found these terms potentially offensive to...

13 August 2023 7,461 0 View

Is there any issue using 12ug/ml of doxycycline for tet/off system?

Hi, I generated a stable cell line with a shRNA. However, when I tested the optimal dose of DOX to silence the target gene I obtained a working concentration at 12ug/mL... So, as the majority of...

07 March 2023 4,725 1 View

Are there any pH indicators emitting in the NIR spectral range?

I'm currently looking for a pH indicator that can be excited in the VIS range (610-630 nm) with emission in NIR range (760-790 nm). I don't have any constrain about the solvent

17 November 2022 6,966 0 View

MrBayes Swap = Lowers ESS?

I am performing mcmc and ss analyzes on MrBayes, with morphological characters, but the ESS are very low. I have already tried to increase the temperature of the swaps and increase the number of...

25 March 2020 4,302 0 View

What is expression of PD-1 on T cell clones during expansion with feeders?

Hi, I would like to know if anyone has expanded CD8 T cell clones with feeders and check PD-1 kinetic of expression. Is it similar to expression on CD8+ cells stimulated with anti-CD3/CD28 beads?...

18 March 2020 6,700 0 View

Where can I access The Greater Cincinnati Well-being Observation Tool?

The Greater Cincinnati Well-being Observation Tool developed by Clarissa Rentz (2005).

15 March 2020 2,728 3 View

What happens to glycerin at 55 °C?

Dear all, Currently, we are trying to produce hydrogen and methane by anaerobic digestion of biodiesel waste (glycerin) at thermophilic temperature (55 °C/ 131 °F). However, as soon as...

10 March 2020 6,805 11 View

Which internal standard should we use in our lactic acid assay?

Hi, We are trying to measure the concentration of lactic acid present in the cervicovaginal samples of pig-tail macaques. Samples were obtained by swabbing the vaginal and cervical tracts....

23 January 2020 9,403 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View