I am looking for a rapid method for evaluation 900 microscopic plant tissue samples. this is my traits under evaluation: in stem: epidermal cells; collenchyma cells; cortex cells; cortex; intravascular sclerenchyma; phloem sclerenchyma; trachea; pith cells. in leaf: epidermal cells; parenchyma cells; trachea; vascular bundles; palisade cells; spongy parenchyma; sheath cells. if anybody suggest a rapid method for staining or a rapid method that do not need any staining like using auto-fluorescence of lignin or anything like this or software for automatic analysis, i`ll be so thankful?
you have to develop experience of observing cells under microscope. You can use fast green and safranin.
Dear Dr Ramawat
Thanks for your attention. I have to develop all my experience at everything in my field certainly but i need more rapid method for automatic recognition of different plant tissue. I know some image analysis software can count cell numbers and other measurements automatically and know some staining dyes like methyl green can stain some particular tissues and some other tissues have auto-florescence that i can use for that software and do my job very fast. But i need some papers or protocols that used that methods systematically. I need some references.
I have noted that the most rapid with nice results method for staining is with toluidine blue which is metacromathic, using the classic Johansen 1940 reference. It can be used in hand made or paraffinized sections. For lignin recognition phloroglucinol and HCl used in non stained and non parafinnized sections (Jensen 1960) .
On the other hand, I do not think there is any automatic method for recognition of plant tissues, because of the great variable nature of these structures (that is why if we want to have a high quality anatomy study, we have to have a lot of patience and experience). If you need to do this work in a rapid way, I would recommend to train some people very well or ask some people with experience to help you with it.
- Badges
- Science method