I am so thankful to Singh and Scanu for providing a suggestion definitely it help me. Dear Singh Sir i will try to download but unable to download so if you have any link so please provide me to download.
Dr A N Singh, you can download XLSTAT 2012 trial version for 30 days, or you can also use XLSTAT 2006 free version. PCA is a type of multivariate analysis. PCA can be easily done by this software. Download it from www.xlstat.com. You can analyse data with in one minute by using it.
Dr A N Singh, you can download XLSTAT 2012 trial version for 30 days, or you can also use XLSTAT 2006 free version. PCA is a type of multivariate analysis. PCA can be easily done by this software. Download it from www.xlstat.com. You can analyse data with in one minute by using it.
The R free software is really appropriate. You need to install the following packages : Rcmdr and factominer. This will make PCA implementation easier.
maybe you have to try using unscrambler program for PCA. it is very easy to work with. also you can do LDA, PLS, SVM and other analysis that are useful for data processing.
With the restriction of free, I would go with R. If you need point-and-click statistics, then something like PAST would work. I have not used PAST enough to really know its limitations.
Dear friends, You guys could use BioVinci for PCA. It is a FREE and powerful web application that produces high quality scientific figures in seconds.
To do the PCA in BioVinci:
Prepare your data matrix (variables in rows)
Upload to BioVinci
Select PCA function
Drag your matrix into the placeholder
Hit Run.
The results comprise of the scatter plot of PC1 and PC2, the bar char of Proportion of variances, and data of all PCs.You could try the PCA example here:
P/S: for high dimension data, the Desktop version of BioVinci is the suitable choice. You could download it here http://vinci.bioturing.com/download-app
If you've limited interest and skills in programming, try OriginLab or MiniTab Software. Both have user friendly interface and you can find loads of video tutorials. Best wishes.
The web-tools like ClustVis and OriginLab software are quite helpful and easy-to-use for the novice in data processing and analyzing. One could upload the raw data and make the PCA plots.
IglooPlot link : https://web.rniapps.net/webigloo/
If you are using PCA also try out this free tool called IglooPlot. It works with the same data input as used for PCA but in many cases allows to get improved insights. The web implementation is extremely interactive to play around and query your data visually as well as perform visual ANOVA on the same. A standalone version of the tool is alo available but the web version is more feature rich.
Web-Igloo visualizes multivariate data in a 2D chart of multiple quantitative variables represented as anchors on a semicircle. The tool not only identifies clusters in your data but also allows to infer the features responsible for formation of the clusters. Projected data points can be easily mapped to class label information using a simple metadata file. View from among a list of examples or upload your own data into our server to generate a customized layout. Web-Igloo plots your multi-dimensional data keeping all the features intact without any feature decomposition.
Highlighted features include
User selectable methods for positioning anchors and selecting normalization options
Options to filter out non informative features
BoxPlot summaries of anchor (or feature) distribution
Interactively visualize sample and feature contributions using bar and bubble plots
Easy mapping of multilevel class label information using a simple metadata file
Convex hull prediction for class boundaries with automated coloring
Simple and flexible search function to simultaneously visualize search outputs in plot as well as metadata table
Interactively identify differentially abundant features across multiple classes using ANOVA
Custom color selection for class labels
IglooPlot link : https://web.rniapps.net/webigloo/
Publication links :
Kuntal BK and Mande SS. Web-Igloo: a web based platform for multivariate data visualization.Bioinformatics. 2017 Feb 15;33(4):615-617.
Kuntal BK, Ghosh TS, Mande SS. Igloo-Plot: a tool for visualization of multidimensional datasets. Genomics. 2014 Jan;103(1):11-20.
Try XLSTAT its very good simple to use. 1 month free subscription there. Moreover youtube has XLSTAT DEMO videos, it will help you a lot to use the software
I made a small free web utility to perform online PCA available here :
https://web.rniapps.net/webigloo/pca/
The data can be uploaded as a tab delimited file along with it's associated metadata (refer to the sample files in the webpage) and visually explore the output.
I have a data from the NGS and I want to do PCOA by using these data. I am having problem in MacQUIIME. If, anyone know any useful information about m query, please let me know. Thank you in advance.
Here https://github.com/gmrandazzo/QStudioMetrics/releases you can find my own software. It is opensource! You do not need to write any line of code to make your multivariate analysis. It works on Linux, OSX and Windows 64 bit.
I tried the https://web.rniapps.net/webigloo/pca/ tool but it was just producing an error. Can you please tell my how many data can be uploaded and how the meta and sample value data have to look?
If you have basic knowledge of Python, work with large data (>10K samples >10K features each) and want to use PCA to reduce the data size, then you may like to try function pca from raleigh package.
pip install --user raleigh
python
>>> from raleigh.interfaces.pca import pca
>>> help(pca) # explains the usage, gives simple usage examples
If you want to analyze metabolomics data by multivariate analyses, such as PCA or PLS-DA, I definitely suggest MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/). It is a free and user-friendly online software. On this website, you can also find an R package named "MetaboAnalystR", in case you want to perform these analyses in R software.