I have been using CTB conjugated with Alexa dyes for retrograde tracing and they work really well. Now we are planning to do some anterograde tracing experiment. Can some suggest a good anterograde tracers.
I've heard that people have had success with PHA-L, but I personally only have experience with CTB.
Would you consider using a viral construct? For example you could use a neuronal promoter (synapsin) driven one for example AAV-syn-ChR2 -YFP/RFP.
I did some sleep recordings and I would like to plot hypnogram for it. How can I plot hypnogram (stages of sleep for 24 hour) with Excel?
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I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
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How can you relate the intensity and red shift of an organic Molecule with its efficiency in DSSC.
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I am attempting to model a Concentrated Solar Power (CSP) model with heliostats and a receiver tower within SolTrace. When altering the heliostat coordinates, the ray tracing indicates that...
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I want to test one sample with multiple fluorescent dyes. Is it possible to premix aliquotes of the sample and the dye and run them in a unstained agarose gel? What is the smartest way for sample...
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I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
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I am planning to study adsorption efficiency of dye at different pH. As we know, some dyes would change colour at different pH. Therefore, is it necessary to plot different calibration curve at...
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I am performing fluorogenic assay with trypsin and fluorogenic assay Z-RR-AMC, my readings are decreasing with increasing time. What could be the reason for this strange results. Please...
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