Sreenivasa Bp

7 Questions 13 Answers 0 Followers

Questions related from Sreenivasa Bp

Can someone share the methodology for syncytial assay?

I googled for syncytial assay protocol. But I couldn't get proper hits for that. If anyone in this forum is aware of or routinely performing syncytial assay, I appreciate if you can share the...

02 February 2015 7,163 2 View

Will pacI undigested recombinant pAdEasy DNA interfere in transfection?

~16ug of Qiagen plasmid mini kit purified recombinant pAdEasy DNA was digested by pacI in 20ul final volume. We used this one (@4ug/well in a 6-well plate) for transfection into 293 cells using...

01 January 2014 6,708 0 View

Which Fetal Bovine Serum (FBS) do you recommend for HEK293 cells?

I request only those researchers who are familiar with culturing of HEK 293 cells to answer this question. In your experience, which FBS is best suited for trouble free culturing of HEK 293 cells?...

12 December 2013 4,059 14 View

Can P-60 be called low passage for HEK-293 cells?

Recently we purchased HEK-293 cell from ECACC. It is supplied at Passage 60. But HEK-293 is available at a passage say 30 elsewhere (e.g., Microbix, Canada). I am just wondering whether we have...

07 July 2013 2,171 4 View

How to get rid of proteins less than 100KDa from a virus preparation? Need a small scale method for this purpose.

I would like to concentrate and purify FMD virus preparation (to get rid of non-structural proteins of less than 100 KDa) . My idea is to initially concentrate the virus by PEG precipitation, then...

05 May 2013 1,085 16 View

Working with Clone Manager for blunt end cloning

Hello, I am learning to use Clone Manager 9.0 for simulation of cloning. I need your help to understand how to simulate cloning of a blunt end PCR product into pCR-Blunt II-TOPO vector. I am...

06 June 2011 9,735 2 View

Protein size discrepancy of His-tagged protein

Hi all, I have expressed a 53KDa His-tagged protein in baculovirus system. The size of the protein is not matching with the expected size i.e 52-56KDa. I am getting the band at 65KDa in SDS-PAGE...

02 February 2010 6,879 8 View