Can someone recommend a vector to construct an infectious clone of a plant virus (7.5kb genome size, has poly A tail)?

24 October 2014 1 5K Report

I am looking to construct an infectious clone of a plant virus for my MSc. Project. the virus I am working with has ssRNA +ve sense genome with a poly A tail and the genome size is ~7.5KB in length. Can someone recommend a vector for me to use?

A.G. Laney

I've used pCB302-3 since it is much smaller than other binary vectors with some success.

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Science method

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