I need to purify total genomic DNA from cervical epithelial cell samples collected by brushing, that are conserved at -20 ° C in a suitable medium.
10.0 ml of 10X CTAB (Hexadecyl trimethyl-ammonium bromide)
10.0 ml 1 M Tris pH 8.0
04.0 ml 0.5 M EDTA pH 8.0 (EthylenediaminetetraAcetic acid Di-sodium salt)
28.0 ml 5 M NaCl
48.0 ml H2O
add 10µl of 2- mercaptaenthol for 1ml of CTAb buffer and incubate at 65C for 10 min
Dear Phani kashyap S:
Many thanks for answering me and sending the information. I really appreciate your kindness.
Kind regards.
Elsa
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