Can anybody suggest mixtures of phospholipids to better mimic bacterial and mammals membranes?

I need to test some peptides by using vesicle models

09 October 2015 891 11 View

Does anyone knows the mechanism of action of the PGRMC1 inhibitor AG-205?

09 October 2015 2,929 1 View

My ZR-75-1 breast cancer cell line grows very very slowly, does anyone have experience with this cell line?

I bought this cell line from ECACC as a frozen sample, and three weeks after thawing its growth is almost undetectable. I'm using the medium recipe recommended.

02 March 2013 9,254 19 View

Is anyone using the 96multiwell microplates GSH coated for immobilizing proteins and detect peptide-protein interactions? Which protocol is the best??

I'm tryng to detect peptide-SH3 domain interaction by using the Thermo GSH coated microplates, but our results are not consistent. Perhaps the buffers we are using are not the best? And how can I...

31 December 2012 6,353 0 View

Same protein found in different spots (different pIs and MWs) in a 2D gel, isoforms?

When I ran a sample of Candida lysate in a 2D gel, I found the same protein in different points of my gel. After LTQ-Orbitrap MS/MS analysis it results the same proteins, and the tryptic fragments...

05 June 2012 9,786 25 View

Does anyone know a method/protocol to allow bacterial ribosome subunits to ri-associate?

01 January 1970 9,750 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View