Alberto Vitali

7 Questions 13 Answers 0 Followers

Questions related from Alberto Vitali

Can someone provide a protocol to study Extracellular matrix in cultured cells by proteomics?

I need a straight protocol to study deposited ECM in cultured cells upon stimuli with selected molecules

09 September 2019 934 2 View

Can anybody suggest mixtures of phospholipids to better mimic bacterial and mammals membranes?

I need to test some peptides by using vesicle models

10 October 2015 1,038 11 View

Does anyone knows the mechanism of action of the PGRMC1 inhibitor AG-205?

I need this information to discriminate the action of AG-205 from that of a peptide

10 October 2015 2,999 1 View

My ZR-75-1 breast cancer cell line grows very very slowly, does anyone have experience with this cell line?

I bought this cell line from ECACC as a frozen sample, and three weeks after thawing its growth is almost undetectable. I'm using the medium recipe recommended.

03 March 2013 9,332 19 View

Is anyone using the 96multiwell microplates GSH coated for immobilizing proteins and detect peptide-protein interactions? Which protocol is the best??

I'm tryng to detect peptide-SH3 domain interaction by using the Thermo GSH coated microplates, but our results are not consistent. Perhaps the buffers we are using are not the best? And how can I...

01 January 2013 6,446 0 View

Same protein found in different spots (different pIs and MWs) in a 2D gel, isoforms?

When I ran a sample of Candida lysate in a 2D gel, I found the same protein in different points of my gel. After LTQ-Orbitrap MS/MS analysis it results the same proteins, and the tryptic fragments...

06 June 2012 9,861 25 View

Does anyone know a method/protocol to allow bacterial ribosome subunits to ri-associate?

I am purifying 30s and 50s ribosome subunits to successively perform studies on their ri-association upon inhibitor addition.

01 January 1970 9,822 6 View