I need this information to discriminate the action of AG-205 from that of a peptide
http://www.ncbi.nlm.nih.gov/pubmed/20144686
I need a straight protocol to study deposited ECM in cultured cells upon stimuli with selected molecules
08 September 2019 979 2 View
I need to test some peptides by using vesicle models
09 October 2015 1,124 11 View
I bought this cell line from ECACC as a frozen sample, and three weeks after thawing its growth is almost undetectable. I'm using the medium recipe recommended.
02 March 2013 9,377 19 View
I'm tryng to detect peptide-SH3 domain interaction by using the Thermo GSH coated microplates, but our results are not consistent. Perhaps the buffers we are using are not the best? And how can I...
31 December 2012 6,494 0 View
When I ran a sample of Candida lysate in a 2D gel, I found the same protein in different points of my gel. After LTQ-Orbitrap MS/MS analysis it results the same proteins, and the tryptic fragments...
05 June 2012 9,900 25 View
I am purifying 30s and 50s ribosome subunits to successively perform studies on their ri-association upon inhibitor addition.
01 January 1970 9,859 6 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...
09 July 2024 6,657 0 View
I am working on my PTM project. I have some peptide. I got the result of doing MD of those peptide without PTM in different force field like Charm-GUI, Amber, Gromacs. Now I have to do MD of those...
05 July 2024 9,346 0 View
I am using a peptide and have faced challenges in preparing a transparent peptide solution. My goal is to prepare a peptide solution in MES buffer and then use the solution to immobilize the...
20 June 2024 8,622 5 View
Dear Researchers, I'm trying to develop the quantification of endogenous peptide in tissue using IDMS as the primary method of characterization. I performed analysis for the calibration curve of...
17 June 2024 9,797 0 View