I want to cross Sarpo Axona with a susceptible variety, and want to determine whether the late blight tolerant gene is available in the crossed product. I want to know how that can be done at the molecular level. Thanks
I am using SU-8 2025 to generate a high aspect ratio microchannel. I made two microchannels with the properties of: 1. width = 10 um. height = 25 um. SU - 8 2025. 2. width = 15 um. height = 37.5...
27 May 2024 6,862 2 View
Hello altruits, would you please help me on how to calculate orthrotropic stress and strain limit in composite simulation in ansys? Thank you so much!
05 March 2024 1,598 1 View
(1)- an international parameter (2)- a free volume (3)- internal, pressure contribution there are three parameter please tells all.
12 February 2024 2,802 0 View
By what measure we can induce cerebral blood flow which can be correlated with wide range of arterial stiffness?
20 July 2023 1,831 2 View
Analysis conducted by Getirana, A. etal. (2022) found that the dry season flow of the river Ganges, Brahmaputra and Meghna has halved: “(O)ur analysis of river-gauge data also shows that the...
23 May 2023 6,677 5 View
I am researching the groundwater availability and related policy responses in Bangladesh in relation to the recent claim of the "Bengal water machine" (Shamsudduha, M. etal.(2022) The Bengal Water...
16 April 2023 2,949 3 View
Requesting help regarding finding the oldest printed copy of Qur’an Ajim Brother, salam and best wishes. I am a lawyer in Dhaka, Bangladesh. I am currently researching the matter of the...
11 December 2022 229 7 View
Dear altruists, I am trying to simulate a transient fluent model with a wall heat source. When I had a udf for heat source, the solution becomes divergent, but when I put constant value, the...
16 July 2022 3,292 0 View
Dea Altruits, I am trying to simulate the CFD simulation of friction stir welding. I have written the code for process simulation. The code is fully compiled. But when I load the code(fiction heat...
09 February 2022 2,108 1 View
Hi, Are there any PD-L1 antibody for use in rats? (For FlowCytometry) Seems as most of the (good) clones only react with human and mouse. Thanks
19 April 2021 6,340 6 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I have designed magnetic material with different biasing conditions in HFSS. Now I want to give an RF AC signal and do a transient simulation in HFSS. Is it possible to do in HFSS? Please help me...
24 July 2024 9,319 5 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View