I have to scan a non-transparent petri dish sized (in term of diameter and thickness) object. The colonies of fluorescent bacteria will be on its upper side. Thus, the UV excitation must come from above.
Does someone know if anyone makes this kind of thing?
Thank you
The Azure Biosystems C600 instrument (and similar instruments from other companies) has fluorescence excitation capability from above the sample. However, the excitation is not UV, but visible light. Several excitation sources are available, with the shortest spectrum in the blue (Cy2).
Thanks a lot,
Have you compared during an in site demo this epi-scanner with models from competitors? The principal issue for me is the sensitivity of the camera and the precision of the autofocus.
thanks in advance
We looked at another brand, and I had previous experience with the Fuji (now GE Healthcare) LAS4000. The Azure instrument seems to me to have competitive sensitivity, and autofocuses correctly. It is also very easy to use. Here is the product brochure.
http://www.azurebiosystems.com/pdf/cSeries.pdf
You could use a microplate reader in fluorescence mode for this. They all excite from above (some also have options to scan from bottom). Just have to adapt your dish to a microplate format
Thanks, Friedrich.
Your suggestion is very interesting, but we have to analyse a whole image of the entire surface of the object. We can't adapt it for microplate format.
Well, you would have to physically make sure, that the dish fits into the reader's plate holder. The measurement stept size then depends on the microplate format youa re telling the reader to use. Liek 1535well plates would be quite some pixels. Some reader also have the possibility to do a "well scan" with high spatial resolution. If you define the round petri dish as one "monster well" the resulting picture will be quite good...
But yes, a camera-based system will make things easier. Depends on what kind of instrumentation is availabe in or around the lab...
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