I am currently looking into reviving T-cells or B-cells from frozen human tissue. Is it feasible? If yes, is there a specific way on how to freeze the tissue in the first place to get the best possible outcome?
This is for immunotherapy-based studies, so in general how a human tissue sample can be stored?
Thank you in advance
Dear Ioannis
Yes you can isolate T/B cells from frozen tissue but they won't be viable unless they were frozen in liquid nitrogen. So if you are after cells for immunocytochemistry or immunohistochemistry, you can do it from a frozen tissue (-20-80C) but if you are after viable cells for functional assays, the tissue should have been stored in correct conditions. Hope that answers your question. Good luck. Siva
Hi,
Please check the link below. This report may contain helpful information about your question.
Best wishes SM Najim
http://www.sciencedirect.com/science/article/pii/S1658387608500409
Dear
I recommend you an interesting mansucript entitled: Reconstitution of paired T cell receptor α- and β-chains from microdissected single cells of human inflammatory tissues. Proc Natl Acad Sci U S A. 2006 Aug 8; 103(32): 12057–12062.
Regards
Marius
A visit to this site may be of help:https://www.stemcell.com/.../BRDX20366-Tools_Immunology_Research.
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