Iv been working with cell lines that iv never worked with before, HUVEC and HT29
the problem started with HT29 then i started seeing in HUVEC also, i would be maintaining the cells throughout the week and even sub-culturing them and they were looking okay with some odd formations (HT29 ->budding or bubble like structures around them) (HUVEC -> long tentacles around some cells)
After the weekend i found most of them dead and detached and the rest are extremely stressed with the budding or bubble like structures around them
This has happened 3 weeks in a row, i changed the incubator since the first time me and a colleague both found our cells dead after a weekend
I didn't change the media since we are running low on DMEM.
Is it contamination or one of the changeable factors is causing this?
Can someone help please?
Hi, Bushra!
The cells may be contaminated with mycoplasma, so it is recommended to perform mycoplasma contamination-related tests. Additionally, hypoxia could also be a possible cause. It is advisable to check the culture conditions in the incubator for further observation.
Hope this helpful. Feel free to reach out for more details.
It could be that the nutrients required for their growth has been depleted.
Try passaging it after three days to a fresh medium. In addition, as another expert has suggested, do not rule out contamination with mycotic cells, a phenomenon that I have personally experienced before.
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