What would be best solvent for polyamides instead of formic acid?

I would like to use solution mixing technique for polyamide and graphene composites. Can I use THF/DMAc/DMSO/DMF? If, so what would be temperature and how much time it may take to dissolve into...

18 January 2021 5,800 3 View

Filtration of graphene oxide?

What is the filtration procedure/filter paper has been used to wash the particle from aqueous solution of hydrogen peroxide followed by HCl aqueous solution if improved hummers has been used ?

22 August 2020 3,054 2 View

How to implement Machine learning algorithm in cloudsim ?

I want to use machine learning concepts in cloud computing. Can any one share learning resource links for implementing Machine Learning algorithms in Java ? I am not aware how to generate or...

09 May 2019 3,417 17 View

Xenograft tumor measurements when using matrigel?

27 October 2016 569 5 View

What is the error associated with runoff estimation from NRCS-CN method in ungauged watershed. e.g a general error percentage.

14 August 2016 8,430 2 View

Anyone familiar with LDH assay and MTT assay?

Hello I run both cell viability using MTT and membrane integrity using LDH analysis, but I find the same compound shows higher levels of LDH release compared to the cell viability data. Can anyone...

15 February 2015 1,206 19 View

Can someone suggest a protocol for preparing cell samples for flow cytometry analysis?

I am trying to find the ROS production in adherent cells after exposing them to stress causing agents, using flow cytometry. I am no sure how should I go about the sample preparation. Please help

14 November 2014 8,200 12 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View