How to compute correctly the heat flux in order to obtain a stable thermal/thermo-hydraulic coupled simulation ?

Dear all, I am trying to couple a simulation with two codes, say code A and code B (different discretizations). For code A we solve simply the conduction equation (solid), while in B we solve the...

24 November 2020 8,961 2 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

How to properly normalize my absolutely quantified mRNA expression?

My PI wanted to use a standard curve to absolutely quantify mRNA expression via RT-qPCR. Her idea was to create the standard curve from a stimulated cell line that expressed the gene of interest...

19 September 2018 4,368 16 View

Is there a specific method to set a Mach Zehnder interferometer for surface relief grating inscription on polymer thin films?

Hello, I have built a Mach-Zhender interferomer in order to irradiate thin films of polymer (PMMA) with an interference pattern. These polymer thin films are doped with metallic precursors, and...

25 May 2016 4,245 4 View

What initiator should I use to prepare a nanocomposite made of Cobalt nanoparticles dispersed in a PMMA matrix?

29 February 2016 6,294 1 View

Calculating the effective mass manually

I am trying to calculate the effective mass of electrons and holes manually from the band structure of a 2D system I am working on. I have only considered the maximum valence band and the minimum...

01 January 1970 4,055 3 View

How to compute correctly the heat flux in order to obtain a stable thermal/thermo-hydraulic coupled simulation ?

Dear all, I am trying to couple a simulation with two codes, say code A and code B (different discretizations). For code A we solve simply the conduction equation (solid), while in B we solve...

01 January 1970 9,673 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View