Can someone help me with the media composition for regeneration of IR64 Rice callus?

24 December 2018 1 10K Report

Is Agaorse, Agar or Phytagel used as a gelling medium and in what concentrations? (in case agarose is used is it the one used in making electrophoresis gel or it is different?)

Wong Ling Chie

We use phytagel 2.5g/L for shoot regeneration

Badges
Science topic

Similar topics
Gels

More Inderjit Kaur's questions See All

How to draw a graphs of bioconcentration of pesticide and its relationship with log Kow, log Sw and molecular weight?

Help me to draw graph of Bioconcentration

10 June 2024 4,941 2 View

How to estimate total sugars using lane and eynon method?

I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...

28 May 2024 9,382 4 View

Dissertation topic for MSC Computer Engineering Students?

My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.

24 May 2024 4,355 2 View

Dissertation Topic for MSC computer Engineering?

Hi, My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.

24 May 2024 1,180 3 View

Why total dissolved solids (TDS) are not reduced after treating waste water by vermifiltration process?

Dear researchers, I am treating the waste water by vermifiltration process using different filter bed media. After the treatment, BOD, COD, TSS were found to be reduced but reduction in total...

18 May 2024 1,897 3 View

What formula we use for calculation of anthocyanin from optical density values?

Using 10 ml of 1% methanolic hcl, 1 g fresh sample and OD at 530 and 657nm

07 May 2024 4,440 1 View

Hello everyone i have some problem when i am installing cooja 3.0 in ubuntu 18.04? give me some solution?

when i am installing cooja 3.0 in ubuntu 18.04 i am facing some problem give me some solution

01 April 2024 4,305 0 View

In the synthesis of nanocomposite material, if amount of components doubles, should the reaction time (blending time) also be increased?

While synthesising nanocomposite material by blending one component over another, it was observed that if amount of components is doubled/increased without increasing blending time (i.e., kept...

19 March 2024 2,781 2 View

Why are my cells dying during puromycin selection after lentiviral transduction?

I have knocked down my gene of interest by lentiviral transduction. I revived the vials and continue growing cells for 72 h without any selection. After confluency, I passaged the cells later 24...

28 February 2024 4,032 1 View

What is 131.25 in formula ABV=(OG−FG)∗131.25?

To calculate ethanol content, we use the formula ABV=(OG−FG)∗131.25 what is this 131.25 constant and how we can calculate it?

19 February 2024 9,034 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View