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Anthracnose that affects most of the horticultural crops is usually caused by Colletotrichum sp. The isolation of the pathogen can be carried out in two ways, Viz Tissue bit culture and single spore isolation. In the tissue bit method the affected leaves are surface sterilised with 3% sodium hypochlorite for 2-3 minutes followed by washing in sterilised water 2-3 times, in LAF . The small lesions along with some healthy portion are cultured on PDA or Water agar. After the visible mycelial growth, the culture is purified by subculturing on PDA in culture plates.
In the single spore isolation, affected fruits are collected and stored in moist chamber until sporulation occurs. Then the spores are collected and cultured on sterilised media.
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