I work with 2D-DIGE and I would like to know about the Samespots (Progeneses). I never worked with this software, because I worked with Melanie (GE). I have problems to analysis my gels.
Thanks, for your answer Binoy Bera.
Hello All! I'm currently doing research on my thesis that i have to extract the data from Twitter and Facebook using certain tags? can someone help me how to do this?
04 January 2021 2,651 4 View
I have a bifactor model with categorical indicators: 4-option Likert-type items. One of my research objectives involves testing measurement invariance between two groups. I have been doing it as...
16 December 2020 4,601 2 View
I have noted dramatically change in color on the body of some marine fishes. For example, I saw a dramatically change from a "intense-yellow" color to "brown" color (normal color), just in one...
19 October 2020 2,717 6 View
I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...
16 October 2020 6,421 2 View
Currently undergraduate student and currently searching for topic that can be used. Can be related to event in the recent 5 years. If have suggestion, kindly comment. Your reply is highly appreciated
17 September 2020 6,879 20 View
Bifactor models allow users to model both a general factor and several specific (or group) factors. The focus is usually on the general factor, which is what the measure was designed for in the...
13 August 2019 9,296 3 View
I made 30 assays testing the capacity of 2 chemical products in the removal percentage of dirt. 15 assays were made with the chemical product `0` and 15 with the chemical product 1. I obtained a...
23 November 2017 8,831 1 View
I'm working on a Spanish version of the 10-item RRS. However, an informal review of published results suggests that the proposed brooding-reflection model shows suboptimal fit. Does anybody have...
04 September 2017 9,877 2 View
I am testing measurement invariance of a questionnaire. I am aware of the fact that some methodologists think it unnecessary to test for invariance of residual variances and covariances. However,...
03 August 2017 284 6 View
There is an increase in the use of bifactor modeling to test for general factors in multidimensional scales. However, its use seems to make sense only when there are high correlations between...
28 May 2017 3,043 2 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View
Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...
16 February 2021 1,808 1 View