How can I adjust weight of temperature and displacement for a least -square objective optimization problem?

Fitting modeling results of a coupled thermal-mechanical numerical model in COMSOL Multiphysics to field data requires solving a least squared objective function which compares temperature and...

29 June 2020 8,533 4 View

The resistance of the sensor was decreased after doping at room temperature why?

the resistance of the sensor was decreased after doping at room temperature why

18 June 2020 5,636 2 View

For one-to-one Y1H expt (CloneTech Takara) should we include stop codon while designing primers for prey cDNA isolation(for cloning in pGADT7) or not?

Dear All We are planning to conduct one-to-one yeast one hybrid experiments. The bait (promoter) is cloned in pAbAi vector and the prey (Transcription factor) is to be cloned in pGADT7. I am...

04 April 2019 2,455 2 View

What is linking factor of GDP?

I have to link India's GDP from 2000-2017, as the base year changes in the time period. Has the statistical department provided with a linking factor for1998-99 to 2004-05 to 2011-12? Otherwise I...

18 June 2018 3,718 1 View

Santa Cruz Flk1 mAb mouse sc393193 gives me a band at 37kDa, does VEGFR2 ever appear cleaved at that mw?

The molecular weight that the bands should show up is at 150 kDa, however a western blot of several cell lines reveal the band at 37kDa uniformly. Could there be any reason why? My literature...

19 May 2018 3,643 2 View

Why do my MDA MB 231 and MCF7 cells exhibit a very different morphology after thawing? Theyve become small and dot like?

Pictures taken at 200x

26 January 2017 2,973 7 View

Can we perform western blot for EYFP attached with channel rhodopsin2 + AAV from the transducted spinal cord samples?

25 September 2014 1,926 1 View

Can someone advise on DNA isolation from DNAzol?

I used Trizol to isolate RNA and then the DNAzol to isolate DNA from the interphase as suggested by the protocol. But the pellet of DNA is so hard that it is difficult to resuspend. Why should...

23 July 2013 5,672 3 View

I am working on ZnS quantum dots, In that to know the exact composition of the sample which characterization can be used?

To know the exact composition of the sample what characterization technique can be used? We don't have access to XPS here can we make use of atomic absorption spectroscopy or XRF? Can you please...

01 January 1970 1,167 15 View

Which characterization tool is most useful in finding out the percentage composition of the dopant used in a compound?

I am working on F doped SnO2 nano particles. My question is that how to know about the exact chemical composition of the dopant. I tried taking EDX but for percentage composition of 5,10 and even...

01 January 1970 1,427 13 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View