The plan is to do a Western blot and our plate reader for measuring protein concentration is not functioning, so we at the lab would like to know an approximate estimate of the total protein concentration that we can get from 1*10^6 Jurkat cells.
Malavika Ramesh
Okay. Since I got no response, I assume not many have an idea regarding this question.
Here goes the answer for the question I posted.
Protein measurements were done using a Nanodrop and a Plate reader (both measurements using BSA standard method).
From a cell concentration of approx. 1*10^7-1.5*10^7 Jurkat cells, we can get about 20-25 mg/ml total protein (lysis using RIPA buffer).
The protein amounts measured by the two machines (Plate Reader and Nanodrop) were comparable and consistent.
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