Can someone explain why 3' overhangs on template DNA produce multiple transcripts in IVT ?

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Asif Nakhuda

The overhangs are needed by DNA polymerase to bind to.

Chanuka Kulatunga

Thanks for the response. Actually in here I used DNA dependent RNA polymerases (SP6 & T7) for the in vitro transcription. not DNA polymerase.

Yes sorry microarray use RNA polymerase for linear amplification which I think is called The Eberwine procedure