Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Can you suggest a good domain identification tool?

I am working on a project to identify sensitive information from text and I am looking into the below categories: -adult content -health -politics -crime Can someone suggest an open source tool...

16 February 2021 4,844 4 View

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

Are the hyperspectral image datasets which are publicly available at ehu.eus website atmospherically and geometrically correct?

I've come across a couple of publicly available hyperspectral image datasets from the website http://www.ehu.eus/ccwintco/index.php/Hyperspectral_Remote_Sensing_Scenes Now, my concern is...

05 February 2021 3,550 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

Are there any fast-acting, long-term fixatives compatible with FISH?

I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...

03 February 2021 7,659 7 View

What are the solvent requirement to study protein conformational changes via NMR?

I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...

31 December 2020 2,103 4 View

Problems in Simulation of Carbon Dioxide discharge from pipe using fluent (Read description below)?

I am trying to simulate carbon dioxide discharging from the pipe using ansys fluent and want pressure variation with time inside the pipe. The geometry consists 144m long pipe and 146.36mm inner...

25 December 2020 1,533 3 View

What are the software using to model for protein?

Hi!! I'm Yedukondalu K, and planing to do in silico analysis for protein. Previous, their is no protein modeling, 3D structure. So, how can I approach to do protein in silico analysis? . I'm...

11 December 2020 9,279 5 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

Should I include irrelevant parts of my methodology?

I am writing up a project in which I used motion capture as well as heart rate monitoring and questionnaires, but I will be analyzing the motion capture data separately to this project write up -...

23 October 2020 6,796 5 View