06 February 2017 4 5K Report

I am looking for lab and field trials with soybean expose to low temperature during emergence and early growth

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Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

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Do I need to correct Sentinel images (_MSIL2A products)?

Hi guys. I have some questions for you. I've seeing a lot of people using sentinel 2A images without atmospheric correction because they say _MSIL2A products are already corrected. However,...

07 December 2020 2,118 2 View

Can anyone help me with a bootstrap resampling problem in TNT?

Greetings, Can anyone help me with a bootstrap resampling problem in TNT? I am currently running an analysis with TNT in a data matrix composed by morphological characters (105 discrete and 25...

23 October 2019 6,645 3 View

Can anyone help me histology TRAP?

Can anyone help me histology TRAP? I'm doing the manufacturer's protocol (SIGMA), and the coloring turns purple. Does anyone know what could be wrong?

09 July 2019 8,894 3 View

Can anyone help me with microplates reading?

I performed assays in microplates for the first time to determine enzymatic activity, but I made a mistake with the reading. The recommended wavelenght for the assay is 550 nm. I used an ELISA...

06 May 2019 3,093 11 View

How to merge two different flowcell of fastq?

Hello, I need to merge two fastq files, which belong to two flowcell, one file has 71 ind and the other 96, so in total they are 167 ind. How can I merge them? I'm analyzing the cyverse data with...

25 November 2018 4,672 3 View

Methods for 3D culture of Hs578T cells?

Hey! I'm currently performing my dissertation using Hs578T cells and I would like to try to perform the mammosphere assay. Has anyone ever worked with this cell line and did this assay? If so,...

18 September 2018 4,619 3 View

What is tRNA level in strain Rosetta (DE3) since it supplies tRNAs for rare codons?

tRNA level in E. coli BL21 strain is ~15% total. I wonder what is tRNA fold increase in strain Rosetta (DE3) since it carries pRARE plasmid.

14 August 2018 5,946 2 View

How to preserve best PBMC samples for performing FACS staining later ON?

08 January 2018 6,965 7 View

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