Can we solate viable cells from frozen xenograft tissues?

I am planning to isolate cells from frozen xenograft tissues (stored in liquid nitrogen). The tissues are stored in 10% DMSO, 30%FBS and 60% RPMI with Penn/Strep. These tissues were frozen with a...

14 December 2020 3,910 1 View

Hello Everyone, Can anyone help me in the transfection procedure of plasmid into chicken spermatozoa by electroporation?

Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....

07 September 2020 7,855 2 View

CG relaxation with B3LYP in VASP?

I am trying to CG relax a amorphous GeSe system with B3LYP and single K-point (gamma). Even though I have a 250 atom model, the calculations seem to be extremely slow. Is there a way to speed up...

23 August 2020 8,848 4 View

Can I use XBridge C18 ( 3x150mm, 3.5 um) column in a Aquity UPLC system for method development?

I have a UPLC system and XBridge C18 ( 3x150mm, 3.5 um). I am running short of time and cant procure a UPLC compatible column for the time being.

15 December 2019 8,433 12 View

Can anybody share links of dorsal hand vein images data set to download?

I need a data set of dorsal hand vein images for experimental purpose.

07 October 2019 1,438 1 View

How to dissolve PtCl2 in water?

HI, I am finding difficulty with the solubility of Platinum (II) chloride. I wanted to prepare PtCl2 in water for electrochemical application.

21 August 2019 5,444 3 View

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We have derived from certain astrometric and cosmological observations how physical constants evolve in time (https://doi.org/10.3390/galaxies7020055). The theoretical basis used is rather...

25 May 2019 8,899 3 View

How to link an enzyme to nanoparticle?

Hi everyone, I am looking forward for a solution to my problem. I want to attach an enzyme covalently on gold nanoparticle surface. If i follow EDC-NHS chemistry, the enzyme-nanoparticle complex...

14 April 2019 7,238 2 View

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Tartaric acid content by HPLC

09 April 2019 3,675 10 View

I have been doing HPLC with 0.1%OPA/MeOH.....after a couple of runs..the pressure started increasing the maximum level @ 40% MeOH?

washed the column in the reverse direction also but the same problem persists. when flow rate is reduced to 0.8mL/min from 1 ml/min...the pressure reduced to 2800 psi apprx. what more can be done...

04 January 2019 7,935 13 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View