I am trying to isolate endothelial cells for culture from afferent mesenteric lymphatic ducts. The afferent lymphatics are the ones going from the intestinal wall to the jejunal lymph node. I manage to visualise and grossly isolate the ducts. However I get left with a lot of surrounding fat. If I trypsinise what I have at the moment I will likely grow a mix culture with endothelial, fibroblasts and a lot of fat cells. I would be grateful if somebody could point out a method to remove the fat cells.
P.S. I work in ruminants, so sorting is unlikely to be feasible. a literature search has not given positive results.
thank you Thomas,
yes a lack of antibody, either for positive or negative sorting is the problem.
Thanks for the UEA I tip, I'll check to see if any cross-reactivity is reported.
best wishes
Mara
thank you again.
I found the protocol you mention in BMC cell biology
linked below in case someone else needs it.
BW
Mara
http://www.biomedcentral.com/1471-2121/8/10
- Badges
- Science method