Why does one set of homologues evolve faster than the other set in an allotetraploid?

Two chromosome sets exist in an allotetraploid, one from the maternal parent and the other from paternal parent. We have an allotetraploid plant species, from which we found that the homeologues...

31 December 2014 4,321 5 View

Which should be used for downstream analysis, contigs or unigenes?

I am dealing with non-reference plant species. I de novo assembled reads into contigs, clustered them based on sequence similarity, and used the longest contig of each cluster as a unigene set. My...

05 June 2014 3,349 1 View

Is there a method to deep sequence one specific gene instead of whole genome/transcriptome?

If there is, is there any commercial service for that, and how much would it cost?

01 February 2014 5,305 1 View

Can I use a small subset of transcripts as a reference for RNA-seq analysis?

Instead of using all the transcripts as reference for the RNA-seq analysis, can I use a small subset which I am interested? I compared the results, of course, the expression values from the small...

08 September 2013 1,361 10 View

Signalp 'Sequence limit reached: Max 10000 sequences are allowed'

Is there an easier way to address this issue except splitting the input file up

08 September 2013 1,472 5 View

Do the tube strips need to be loaded evenly on the themocycler of StepOnePlus?

Do the tube strips need to be loaded evenly on the themocycler of StepOnePlus?

06 July 2013 5,499 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

No title

23 February 2021 4,014 3 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View