Is there a method to deep sequence one specific gene instead of whole genome/transcriptome?

02 February 2014 1 5K Report

If there is, is there any commercial service for that, and how much would it cost?

Yes, you are very right. But the reason I want to do this is not that I want to evade the bioinformatic analysis, but try to find a cheaper and ideal way to fulfill my goal. Since I am dealing with allo-multiple-ploidy species, it is tedious to clone individual homeologs or homologs, and then sanger sequence them, but it would be too costly to use whole transcriptome/genome sequencing, since I am just interested in that specific gene. So what was on my mind is similar to what you suggested, I will barcode the sequence I want with unique sequence for each sample, and I pool all my samples into one library, so I can use just one deep sequencing to do all my samples. I want to know if there is anyone who has done anything similar before and has a mature protocol that I can follow.

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