Shu Chen

7 Questions 13 Answers 0 Followers

Questions related from Shu Chen

Why does one set of homologues evolve faster than the other set in an allotetraploid?

Two chromosome sets exist in an allotetraploid, one from the maternal parent and the other from paternal parent. We have an allotetraploid plant species, from which we found that the homeologues...

01 January 2015 4,340 5 View

Which should be used for downstream analysis, contigs or unigenes?

I am dealing with non-reference plant species. I de novo assembled reads into contigs, clustered them based on sequence similarity, and used the longest contig of each cluster as a unigene set. My...

06 June 2014 3,364 1 View

Is there a method to deep sequence one specific gene instead of whole genome/transcriptome?

If there is, is there any commercial service for that, and how much would it cost?

02 February 2014 5,319 1 View

Can I use a small subset of transcripts as a reference for RNA-seq analysis?

Instead of using all the transcripts as reference for the RNA-seq analysis, can I use a small subset which I am interested? I compared the results, of course, the expression values from the small...

09 September 2013 1,379 10 View

Signalp 'Sequence limit reached: Max 10000 sequences are allowed'

Is there an easier way to address this issue except splitting the input file up

09 September 2013 1,491 5 View

Can someone advise on how to dissolve 24-epibrassinolide?

I use 24-epibrassinolide from PhytoTech. On the MSDS, it only give its solubility in DMSO. However, all the paper I read dissolved it by Ethanol. Is there anyone ever used 24-epibrassinolide of...

07 July 2013 5,064 2 View

Do the tube strips need to be loaded evenly on the themocycler of StepOnePlus?

07 July 2013 5,534 1 View