I have got Apyrase 14.9 g solid, labelled as 3-40 Units/mg protein. Does anyone know about the working concentration of the compound to lower the baseline signal in ERK activation assays; to be used for Western blotting or In-Cell Western?
With the Qubit High Sensitivity DNA kit, I was measuring 8 samples (2uL each + 198 of the working solution), and noticed that 4 of the 8 samples had readings of >60ng/uL. I then re-did the...
22 April 2024 7,183 1 View
I am trying to use the NAMD Energy plugin, but am repeatedly faced with the error "FATAL ERROR: Unable to access config file namd-temp.namd" I have tried moving the relevant files to the working...
09 January 2024 6,565 0 View
I am trying to use the BFEE Plugin to calculate Free Energy of a protein-ligand complex. I generated the relevant RMSD, Theta, Phi, etc folders and files but am unsure how to proceed? The...
09 January 2024 3,484 0 View
Editorial manager (And Manuscript Central) now forces LaTeX submissions to be submitted as raw LaTeX files, which sounds easier than it is. After spending many hours figuring this out, I thought...
25 July 2023 3,239 0 View
Hello, could someone help on how to interpret an IndVal value? I have run an Indicator Species Test in PAST (I have no Primer and R doesn't take missing values, I was only able to run my data in...
10 July 2023 6,348 1 View
Hello, does anybody know of any program that implements SIMPER and IndVal on a dataset with many missing values? I know that PAST implements SIMPER but with column average substitution for missing...
07 July 2023 2,143 0 View
Hello. I am wondering if anyone will point me to any resources on how I may conduct SIMPER analysis for non-parametric data. I have around 50 variables but many of my groups have few N samples...
27 February 2023 3,176 3 View
As anyone done alpha-glucosidase inhibition assay for non polar compounds which are not soluble in DMSO and buffer it is soluble in hexane ethylacetate and toluene ?
17 January 2023 5,013 0 View
Why do many researchers normally use ultrasonic bath generating random stress for piezocatalytic experiment? What's the difference between random stress and random strain? Does the...
14 March 2022 4,264 0 View
I want to optimize geometry of molecule with constraints. Can I set a constraint box to confine a molecule? For example, when a box is too smaller than the molecular size, the total energy might...
30 October 2021 9,182 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View