I am working on fake news detection. Where can I get fake news dataset?

I am working on fake news detection system that published in newspapers and/or facebook. How and where can I get fake news dataset to train my model?

10 November 2020 5,072 5 View

65mn Spring Steel Bainite vs Martensite Structure properties ?

Hello I have encountered one problem . I am using 65mn steel material for one of my product as spring part which will get deformed in application load. I am seeing the part getting into plastic...

17 August 2020 9,501 3 View

Cantilever beam with heavy mass and spring at the free end. How to configure the spring in Ansys?

Hey, I'm trying to find the frequencies of a cantilever beam with heavy mass and spring attached at the free end as shown in figure. First, I found the solution without the spring and it was...

07 July 2020 9,675 3 View

Can anyone suggest an open source tool for Sensor error correction in Hyperspectral Image processing?

open source tool for Sensor error correction in Hyperspectral Image processing

28 January 2020 6,426 2 View

Urease SDS PAGE?

Urease enzyme has six subunits and its molecular weight is ~544620 Da. One subunit is about 90700 Da. How to electrophoresis of urease with SDS (SDS-PAGE)?

26 November 2019 6,575 4 View

TiO2 nanotube synthesis?

These days, I have tried to synthesize TiO2 nanotubes but failed again and again. I attached SEM images of as-synthesized TiO2 samples. (Most parts of TiO2 sample were not made to nanotubes (1)....

20 November 2019 4,312 4 View

TiO2 nanotube anodization..?

I'm trying to prepare TiO2 nanotubes by anodizing Ti metal. Electrolye is 0.5 M H2SO4 and 0.5% HF and cathode is SUS. Anodization for TiO2 nanotube synthesis usually takes about 2 h. And it is...

26 June 2019 2,103 3 View

Synthesis of TiO2 nanowires?

I used to synthesize TiO2 nanowires from a Ti plate through a wet corrosion method: (1)dipping a Ti plate in a KOH solution (10 M) for 24 h at room temperature, (2) dipping a KOH-treated Ti plate...

13 June 2019 1,468 3 View

MPC & Learning Based MPC, Definition and Novel Ideas ?

Regarding recent advances in MPC, What kind of novel ideas can be made on exploiting MPC to issues, e.g. optimal & On-line trajectory design for Aerospace vehicles? I need to know what is...

04 February 2018 4,139 6 View

Which post-hoc test among DMRT and Bonferroni is the best for multiple comparison analysis? Can we carry on both post-hoc tests?

07 September 2016 1,848 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View