My glycerol stock bacteria does not grow?

I made 30 bacteria transformed with plasmid including ampicillin resistant gene. transformed bacteria (total 30) were mixed with 25% glycerol and stored in -70 defreezer ----> stock : 500 ul...

04 May 2019 3,118 2 View

Is there good publication showing red pepper cell structure?

10 November 2016 7,550 0 View

How does a filamentous fungi get foreign genes?

In transformation experiment of M. oryzae, when I mix any genes with m. oryzae protoplasts, they are easily transformed by the genes. I want to know how they get foreign genes. Do they get the...

01 February 2016 5,065 1 View

Do you know about Fusarium sexual stage and asexual stage?

31 December 2015 9,348 1 View

In fungi, where are the proximal hyphal compartment and distal hyphal compartment?

08 September 2015 6,613 1 View

Any advice on the AFLP amplification step?

05 June 2015 6,501 6 View

Any advice on a white pellet in the lysis solution?

05 June 2015 2,413 3 View

What is degenerate cell?

Hello. I am reading a book that deals with virus evolution. In hypotheses on the origin of viruses, there is a sentence " Viruses are derived from degenerate cells that eventually parasitised...

04 May 2015 348 1 View

How can I make a 50pmol/ul EcoRI adaptor?

03 April 2015 8,316 1 View

How can I solve my protoplast generation problem?

01 February 2015 3,057 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View