I'm looking for a good image analysis software beyond of what imageJ can do. Specifically I'm looking at advanced Calcium imaging analysis and 3D reconstruction and analysis. Any opinions/experiences with Volocity or Aivia? Any other software options I should consider? Primarily I'm looking at the advanced high profile software that runs pretty expensive, such as Volocity or Aivia, but free more simple apps can be considered in addition.
Thank you for your response!
Dear Madina,
there is a strong Image Processing Tool in Matlab and it is transparent (from scientifical point of view). Before recommend any further software it would be helpful to know what kind of information would you like to extract from images or how the images (I suppose 2D) are arranged for 3D reconstruction?
Regards
Dear Helene,
My primary interest is analysis of 2D time-lapse Calcium imaging datasets. Here I'm looking for an automated ROI selection and analysis of Calcium fluctuations with respect to time. For 3D reconstruction (EM and confocal images) we have a stack of Z-planes with a set z-step size between images. Tracing and object identification is something we are looking for from a 3D reconstruction.
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