Can some one kindly provide me the protocol for extracting antioxidants from micro algae (chlorella, chaetoceros, nanochloropsis )?

More Sharareh Savari's questions See All

Abaqus-XFEM-SIF results convergence?

Dear all, I have extracted SIF results based on XFEM analysis for a stationary crack in 6 contours, as depicted in the attached graph; however, it is unclear whether the SIF value has converged....

29 July 2023 7,281 0 View

Precision of Geometry in Abaqus?

Hello everyone! I have recently started using Abaqus. I am attempting to model a small crack in a spirally-welded pipe. However, I'm facing difficulties while using the contour integral method to...

10 July 2023 2,210 2 View

What is the difference between implementation science and community-based participatory research?

I am a health services researchers with expertise in systems science and GIS methods. My research is focused on identifying areas in need of specific interventions that can address food insecurity...

23 January 2023 4,236 3 View

Is there a theoretical framework for health-related interventions in community resources?

I am looking for a conceptual framework/theory that has been the basis of health-related interventions in community resources (e.g., parks, schools, pharmacies, churches). My research will inform...

28 September 2022 7,715 3 View

Can I recode the Categorical variables in surveys?

I am analyzing a Nationally representative survey and I wonder if I recode the categorical variables like gender or education, it would mess the weights! each row of the data has a weight,...

15 December 2021 3,096 8 View

How to Interpret the results of a Geographically Weighted Regression model for a categorical independent variable?

I am running Geographically Weighted Regression (GWR) on a categorical dependent variable so my model is basically a Geographically Weighted Logistic Regression. I have multiple independent...

09 December 2021 2,088 2 View

What is the best protocol for the extraction of EPS (Exopolysccharides) of bacteria?

I am looking for a protocol to prepare high yield of EPS.

13 December 2018 5,801 3 View

What are the general factors that may affect enzyme production by bacteria?

I mean general hydorlytic enzymes like cellulase, protease, etc.

08 May 2018 9,059 7 View

What is the difference in hydrodynamic properties of liposomes made using thin film hydration method and ethanol injection method?

Hi , I am looking for a study that compared properties of liposome made using thin film hydration method with the liposome made using ethanol injection method having the same components. Have you...

22 November 2017 1,586 0 View

What are biochemical tests used for gram positive and gram negative thermophilic bacteria?

I mean what biochemical tests are routinely used for identification of thermophilic bacteria in a lab such as OF, MR-VP, etc.

04 October 2017 7,860 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View