Temp, time, pH, C and N source are the main factors which

affect enzyme production .

Regards

Abhishek Sharma

Hi Harichi,

There are many factors which affect to the enzyme production of bacteria. The factors include pH, temperature, components of medium, conditions of culture, agitation, aeration, DO, time of fermentation. Especially, enzyme production heavily depends on strain of bacteria.

Best Regards.

Phúc Tô Đình,

Hi

thanks for your reply. If for example you have the bacillus related genera like Aeribacillus that produces xylanase.

Hi Harichi,

There are many factors which affect to the enzyme production of bacteria such as pH, temperature, sole carbone source that provided for enzyme production, agitation and aeration.

The pH and temperature are key factors as contributed by other researchers. However the concentration of substrate (carbon source) may also contribute. Some bacteria produce certain enzymes in the presence of excess carbon source. So try varying the concentration of carbon source in your experiments. Cheers

Temp, time, pH, C and N source are the main factors which

affect enzyme production .

Regards

Abhishek Sharma

The general factors that may affect enzyme production by bacteria include the following: temperature, pH, inoculum size or concentration, substrate type and particle size, the type of solvent used for extraction, agitation during the process of enzyme recovery from the substrate, among many other factors listed above.

It has been however proven from literature that, bacteria require more moisture than fungi during fermentation hence, the need for submerged fermentation for enzyme production with bacteria.