I need to quantify pyruvate levels in tissue samples. However, endogenous enzymes in tissues such as LDH deplete pyruvate. Deproteinization by spin columns is slow and precipitation and neutralization is tedious and not accurate.

Best way is to heat samples to denature all enzymes and then centrifuge. I have already applied this step to measure lactate. However, I am not sure that pyruvate is as stable as lactate. I was wondering if somebody has used heating step for the assay of pyruvate using kits? or if someone has information that pyruvate is as thermostable as lactate?

Thanks