What length of long non coding RNA (lncRNA) should be used for its overexpression in plants?

Hi all, I am planning to over-express a lncRNA (under 35S) in Arabidopsis. Will the presence of 20-30 bp sequences upstream and downstream of lncRNA sequence affect its over-expression? I...

07 August 2019 6,875 1 View

Is there any online tool to make delta SNP index plots?

HI Research family, I am trying to construct delta SNP index Manhattan plots. Are there any stand-alone or online software for it! I am looking for something that doesn't involved programming....

04 May 2019 2,886 4 View

What should be the path length when doing enzyme assays in 96 well-microtiter plates?

06 July 2017 9,565 9 View

Over-expression of pre-miRNA not translated into increased expression of mature miRNA?

Hi everyone, I have cloned precursor sequence of a miRNA (say mIR1) in Arabidopsis and generated three independent, homozygous transgenic lines in Arabidopsis. When I performed qRT-PCR of these...

03 April 2017 5,134 15 View

How can I normalize the sequencing data in two different libraries?

Hi Everyone If I have data from four miRNA libraries. One of the libraries have 4 times more reads than other three libraries. How will the data be normalized among different libraries? People use...

02 March 2015 1,893 8 View

What is the difference between over-expression of miRNA and Artificial miRNA technology?

02 March 2015 6,218 11 View

How can I find the 1kb upstream region of miRNA precursor gene in Brassica napus?

Hi Everyone, I am trying to find stress responsive cis elements in the upstream regions of miRNAs in Brassica napus. miRbase gives me the genomic coordinates of miRNA precursor gene, but I am not...

11 December 2014 5,414 2 View

Can housekeeping genes (actin, GADH,UBC9 etc.) be used as an endogenous control for plant miRNA qPCR?

Hi Everyone I have tried using U6 and 5.8sRNA as endogenous control. Although the expression of both these remain constant in different conditions, the Ct values for U6 are quite high (30 or so)...

10 November 2014 9,974 5 View

What does the +/- sense of orientation actually mean and what is its implication in determining miRNA precursors in plants?

I have a number of miRNA precursors. These are the ones that mapped to a concatenated genome consisting of B. napus EST, B. oleracea EST and B.rapa genome. Some of the precursors have mapped to...

10 November 2014 5,073 1 View

How should I interpret plant miRNA data? When doing clustering of miRNAs in families which sequence should I use?

Hi everyone I am doing a next gen sequencing project to identify miRNA in brassica. I made the small RNA libraries and sent them for sequencing and subsequent analysis. The data that I got back...

08 September 2014 9,817 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View