What length of long non coding RNA (lncRNA) should be used for its overexpression in plants?

Hi all, I am planning to over-express a lncRNA (under 35S) in Arabidopsis. Will the presence of 20-30 bp sequences upstream and downstream of lncRNA sequence affect its over-expression? I...

07 August 2019 6,875 1 View

What should be the path length when doing enzyme assays in 96 well-microtiter plates?

06 July 2017 9,565 9 View

Over-expression of pre-miRNA not translated into increased expression of mature miRNA?

Hi everyone, I have cloned precursor sequence of a miRNA (say mIR1) in Arabidopsis and generated three independent, homozygous transgenic lines in Arabidopsis. When I performed qRT-PCR of these...

03 April 2017 5,134 15 View

How can I normalize the sequencing data in two different libraries?

Hi Everyone If I have data from four miRNA libraries. One of the libraries have 4 times more reads than other three libraries. How will the data be normalized among different libraries? People use...

02 March 2015 1,893 8 View

What is the difference between over-expression of miRNA and Artificial miRNA technology?

02 March 2015 6,218 11 View

How can I find the 1kb upstream region of miRNA precursor gene in Brassica napus?

Hi Everyone, I am trying to find stress responsive cis elements in the upstream regions of miRNAs in Brassica napus. miRbase gives me the genomic coordinates of miRNA precursor gene, but I am not...

11 December 2014 5,414 2 View

Can housekeeping genes (actin, GADH,UBC9 etc.) be used as an endogenous control for plant miRNA qPCR?

Hi Everyone I have tried using U6 and 5.8sRNA as endogenous control. Although the expression of both these remain constant in different conditions, the Ct values for U6 are quite high (30 or so)...

10 November 2014 9,974 5 View

What does the +/- sense of orientation actually mean and what is its implication in determining miRNA precursors in plants?

I have a number of miRNA precursors. These are the ones that mapped to a concatenated genome consisting of B. napus EST, B. oleracea EST and B.rapa genome. Some of the precursors have mapped to...

10 November 2014 5,073 1 View

How should I interpret plant miRNA data? When doing clustering of miRNAs in families which sequence should I use?

Hi everyone I am doing a next gen sequencing project to identify miRNA in brassica. I made the small RNA libraries and sent them for sequencing and subsequent analysis. The data that I got back...

08 September 2014 9,817 3 View

How important are A260/A230 and 28S/18s ratios for RNA that are to be used for miRNA deep sequencing?

I did an RNA isolation by TRIzol method from canola plants. When measured by NanoDrop 1000 the A260/A280 ratios for RNA were between 1.7-2.0 while the A260/A230 ratios were between 0.7-1.5. Though...

07 August 2013 6,684 35 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

How to find fast publication Scopus Indexed (Q2) journals in Computer Science and Engineering?

Dear Researchers, I would like to find some (Q2) Scopus-Indexed journals with fast publication in the field of Computer Science and Engineering major?

28 February 2021 3,285 2 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Scopus Index Journal List , 2021?

please provide me the most recent Scopus Index Journals List, February-2021

27 February 2021 3,545 1 View

How can be researcher's index developed?

What are the criteria to be followed to develop the researcher's index?

26 February 2021 9,051 0 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View