How to purify Plasmid DNA after RNAse treatments and Restriction enzyme digestion for high yield Capped RNA Transcription ?

I tried phenol/chloroform purification method with 2 microgram DNA after RNAse and Restriction enzyme digestion but I am not getting enough quantity of plasmid DNA at the end. I am just getting...

25 September 2020 7,591 4 View

I am doing for Protein-ligand MD simulation using Gromacs but facing some problem during Ligand Topology step. How to resolve it ?

after running python3 cgenff_charmm2gmx_py3_nx2.py LIG lig_fix.mol2 lig.str charmm36-jul2020.ff I am getting following error: error in atomgroup.py: read_mol2_coor_only: no. of atoms in mol2 (44)...

30 August 2020 4,546 3 View

Any one tell me, which is the propel dose of alloxan mg/ kg to induce diabetic in rat wighted 200-250 g .?

01 January 2017 8,145 3 View

Any one answer me, when and how clomid is used for male infertility?

31 December 2016 8,190 0 View

Any one tell me, what are the side effects of using Cyproterone ...is it true that ,daily usage lead to depression .?

needs more informations

31 December 2016 4,661 0 View

Do you think that the psychological factors often play a bigger role in creating sexual dysfunction,in both women and men. ?

31 December 2016 7,713 0 View

May i know chemical material that can run out to the placenta to induce congenital abnormalities and changes in the fetus Genet?

31 December 2016 1,978 1 View

Has anyone used SYBR Gold in a homemade qPCR mix?

I have tried adding 1X SYBR Gold to a PCR mix (Hot Start Qiagen) with primers and DNA that I know work with a normal PCR. However, I did not get any amplification curve (detection at 550nm), nor...

20 August 2015 7,335 3 View

Need to know the chemical reactions of convention the precursor proacrosin to acrosin in sperm head during the acrosome reaction.?

01 January 1970 3,523 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View