Safa Naji

3 Questions 3 Answers 0 Followers

Questions related from Safa Naji

What's the meaning of a melting curve that starts with a peak in rt_pcr??

I used primers for a house keeping gene and gene of interest, the result showed in attached picture

28 November 2021 8,223 3 View

What if I used hot wash1 solution during bacterial RNA extraction by trizol method?

During RNA extraction I used preheated wash 1 solution( about 40C) "by mistake", discarded the filtrate, used wash2 and then the preheated elution buffer (the only buffer that should be heated...

09 November 2021 2,242 3 View

Can PCR with specific primer of bacterial genus yeilds results using another bacterial genus?

What is the meaning of having results when using a specific primer for E.coli gene, for example, in klebsiella pneumonia.. The gene product is present in both, but is it normal that the gene is...

03 August 2021 7,778 5 View