Hello!

I wish to perform prime editing from A->G (upper strand) on induced pluripotent stem cells. However, as the 5' of my target editing region does not have PAM sites, would the figure I have illustrated be possible for me to perform prime editing?

I have not really seen this design documented in papers hence I was wondering if there was anything I was missing. I made sure that all major criteria such as 5' synthesis and 5' endonuclease flap are fulfilled.

Alternatively, I am really debating between prime editing and ABE (adenosine base editing), and will really appreciate if anyone could share their thoughts as to which technique is more suitable for my surrounding sequences :) Right now, I am leaning towards ABE due to it's higher efficiency and lower indel formation. However, I am not too sure due to the presence of multiple As around my target region :(

I would really appreciate any comments or help, thank you so much!