What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Measurement invariance of a bifactor model with categorical indicators. What is the recommended approach?

I have a bifactor model with categorical indicators: 4-option Likert-type items. One of my research objectives involves testing measurement invariance between two groups. I have been doing it as...

16 December 2020 4,601 2 View

How to concentrate a PCR product before sequencing?

Hi everybody, I'm planning to sequence several phytoplasma strains by doing a nested PCR and cloning the resulting amplicons. Standard phytoplasma universal primers pairs will be used (P1/P7...

08 November 2020 2,155 4 View

Discrepancy protein levels and receptor expression?

I measured the expression of a protein that is a receptor in a panel of cell lines. When I measured the protein levels by Western blot the protein levels were about the same in all cell lines ,...

09 September 2020 6,671 3 View

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24 July 2020 9,872 3 View

How can I design an experiment capable of demonstrating if my cells can grow without a substrate?

Dear Researchers, I am incredibly confused about the in vitro colony forming assay. I was asked to perform such assay to assess my cell models capability to grow without attachment to a solid...

10 June 2020 6,230 3 View

Who decides if a paper is submitted or not?

If one of the coauthors of a research paper does not contribute intellectually but financially only to a project, is he/she entitled to call off the submission of the paper?

10 May 2020 2,851 7 View

Does anyone know home range databases for mammals and birds?

I am conducting a study using wildlife traits, however, home range data is limited. Pd. I have already used data from Tamburello et al. 2015, but I still have lots of species without home range data.

30 April 2020 6,608 2 View

IEEE 34 node test feeder in DigSilent PowerFactory?

18 April 2020 1,949 2 View

Can pressured steam carry small biomolecules?

Apparently there is a belief that contaminated steam in the autoclave can contaminate your pipette tips while sterilizing them with biomolecules such as peptides and DNA, and that this can be a...

05 March 2020 1,439 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View