There is some controversy regarding NIH-3T3 due to its tumorigenicity and metastatic potential under certain conditions. If so, design of experiments to evaluate therapeutic potential by comparing cytotoxicity of cancer cells with NIH-3T3 (as normal cells) seems not only far-fetched but also completely fallacious. Also, is it justifiable to use NIH-3T3 as a model to represent the effect of a particular therapeutic agent on normal human cells?
Absolutely not!
Any and every cell that has been isolated from a living organisms cannot be considered to be normal. The longer it is in culture the further removed it is from 'normal'.
3T3 cells have been around for so long they are equivalent to a new life form!
DIL
No, they gained their unique properties through the cell culture process.
http://www.nih3t3.com/
http://www.asbmb.org/asbmbtoday/201501/Generations/3T3/
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