Can Naegleria fowleri undergo apoptosis by intrinsic pathway of apoptosis?

02 February 2014 1 4K Report

Not all Eukaryotic PCD are susceptible to apoptosis. Does this organism have the requirements to go into apoptosis is the question?

Sharon Frentzel

I'm sorry, I do not have information to assist with your question.

Badges
Science topic

More Abdul Mannan Baig's questions See All

Does human stable and permanant cell genome contain a stem cell regeneration regulating site?

This activation can self renew the damages on injury, unlike induction of OCT4 and other iPS inducing genes. Cell can be programmed to self activate them on stimulus.

11 December 2016 6,857 3 View

What functions, other than participation in protein synthesis, are performed by aromatic ring amino acids, in the biology of Acanthamoeba ?

Acanthamoeba has a specific pathway for the synthesis of aromatic ring amino acids. These amino acids like other 16-17 amino acids participate in synthesis of macromolecules like proteins and...

05 June 2015 6,182 4 View

What are your views on the issue of Stem Cells and Obokata?

The hot topic of stem cells these days, with Obokata retracting her papers published in Nature. There was a simple question for reviewers from the very first day. Why would an acid exposure ( a...

06 July 2014 3,126 1 View

Has anyone ever heard of a case of Naegleria Meningoencephalitis with CSF or Bloody Rhinorrhea?

As upper part of nasal cavity (olfactory bulb and Cribriform plate) are invaded by Naegleria before spreading to the adjacent inferior surface of frontal lobe of brain, a CSF or/and a bloody...

01 February 2014 5,538 6 View

Is there any study on primitive GPCRs, the ones that have multiple docking pockets for different ligands?

In Acanthamoeba castellanii, I have come across some GPCRs that are unique primitive ones with more than one docking pockets. I was wondering if any other unicellular eukaryote has similar...

01 January 1970 2,956 1 View

How did so much microbial DNA got incorporated in human chromosomes?

Human chromosomes have a very vast amount of microbial DNA incorporated in them, I was wondering how it made its way here? Lateral gene transfer? Our evolution occurred from a unicellular stage to...

01 January 1970 8,642 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View