Does human stable and permanant cell genome contain a stem cell regeneration regulating site?

This activation can self renew the damages on injury, unlike induction of OCT4 and other iPS inducing genes. Cell can be programmed to self activate them on stimulus.

11 December 2016 6,726 3 View

What functions, other than participation in protein synthesis, are performed by aromatic ring amino acids, in the biology of Acanthamoeba ?

05 June 2015 6,055 4 View

What are your views on the issue of Stem Cells and Obokata?

The hot topic of stem cells these days, with Obokata retracting her papers published in Nature. There was a simple question for reviewers from the very first day. Why would an acid exposure ( a...

06 July 2014 3,000 1 View

Has anyone ever heard of a case of Naegleria Meningoencephalitis with CSF or Bloody Rhinorrhea?

As upper part of nasal cavity (olfactory bulb and Cribriform plate) are invaded by Naegleria before spreading to the adjacent inferior surface of frontal lobe of brain, a CSF or/and a bloody...

01 February 2014 5,410 6 View

How did so much microbial DNA got incorporated in human chromosomes?

Human chromosomes have a very vast amount of microbial DNA incorporated in them, I was wondering how it made its way here? Lateral gene transfer? Our evolution occurred from a unicellular stage to...

01 January 1970 8,507 5 View

Is there any study on primitive GPCRs, the ones that have multiple docking pockets for different ligands?

In Acanthamoeba castellanii, I have come across some GPCRs that are unique primitive ones with more than one docking pockets. I was wondering if any other unicellular eukaryote has similar...

01 January 1970 2,813 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View