Does human stable and permanant cell genome contain a stem cell regeneration regulating site?

This activation can self renew the damages on injury, unlike induction of OCT4 and other iPS inducing genes. Cell can be programmed to self activate them on stimulus.

11 December 2016 6,726 3 View

What functions, other than participation in protein synthesis, are performed by aromatic ring amino acids, in the biology of Acanthamoeba ?

05 June 2015 6,055 4 View

What are your views on the issue of Stem Cells and Obokata?

The hot topic of stem cells these days, with Obokata retracting her papers published in Nature. There was a simple question for reviewers from the very first day. Why would an acid exposure ( a...

06 July 2014 3,000 1 View

Can Naegleria fowleri undergo apoptosis by intrinsic pathway of apoptosis?

Not all Eukaryotic PCD are susceptible to apoptosis. Does this organism have the requirements to go into apoptosis is the question?

01 February 2014 4,317 1 View

Has anyone ever heard of a case of Naegleria Meningoencephalitis with CSF or Bloody Rhinorrhea?

As upper part of nasal cavity (olfactory bulb and Cribriform plate) are invaded by Naegleria before spreading to the adjacent inferior surface of frontal lobe of brain, a CSF or/and a bloody...

01 February 2014 5,410 6 View

How did so much microbial DNA got incorporated in human chromosomes?

Human chromosomes have a very vast amount of microbial DNA incorporated in them, I was wondering how it made its way here? Lateral gene transfer? Our evolution occurred from a unicellular stage to...

01 January 1970 8,507 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View