I am doing RNA and DNA extraction using the basic phenol / chloroform / isoamyl method to quantify RNA and DNA in aqueous phase, but first, I do a lysis with buffer (Tris-HCL 1M pH8; EDTA 0.5M pH8 and SDS 1%) . Could this buffer interfere with quantitation? * quantification by Qubit
Sure, dependent on the ingredients (detergences), isolation/lysis can affect quantification (e.g. via a Bradford-based method).
I don't think the ingredients in your lysis buffer will affect Qubit quantification.
Article Pitfalls of DNA Quantification Using DNA-Binding Fluorescent...
http://www.science.smith.edu/cmbs/wp-content/uploads/sites/36/2015/09/Qubit-dsDNA-HS-Assay.pdf
Read page 7 and 8 of dsDNA qubit manual (link given above) which discusses the possible contaminants and amount of variation expected.
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